Quantitative proteomic tools for analyzing histone modifications
نویسندگان
چکیده
منابع مشابه
Quantitative Proteomic Approaches to Studying Histone Modifications
Histone post-translational modifications (PTMs) positively and negatively regulate gene expression, and are consequently a vital influence on the genomic profile of all eukaryotic species. The study of histone PTMs using classical methods in molecular biology, such as immunofluorescence and Western blotting, is challenging given the technical issues of the approaches, and chemical diversity and...
متن کاملRecent Advances in Maize Nuclear Proteomic Studies Reveal Histone Modifications
The nucleus of eukaryotic organisms is highly dynamic and complex, containing different types of macromolecules including DNA, RNA, and a wide range of proteins. Novel proteomic applications have led to a better overall determination of nucleus protein content. Although nuclear plant proteomics is only at the initial phase, several studies have been reported and are summarized in this review us...
متن کاملKnotting the NETs: Analyzing histone modifications in neutrophil extracellular traps
than a decade since their discovery, neutrophil extracellular traps (NETs) remain in the headlines. Initially, interest focused on the structure of extracellular NET chromatin and its capacity to capture and damage bacteria. Soon, however, researchers began to see the impli cations of extracellular chromatin for the development of autoimmune diseases. One quintessential auto-immune disease, sys...
متن کاملProteomic databases and tools to decipher post-translational modifications.
Post-translational modifications (PTMs) are vital cellular control mechanism, which affect protein properties, including folding, conformation, activity and consequently, their functions. As a result they play a key role in various disease conditions, including cancer and diabetes. Proteomics as a rapidly growing field has witnessed tremendous advancement during the last decade, which has led t...
متن کاملP-98: Effect of Mouse Embryo Vitrification on Histone Modifications
Background: Vitrification has been usually used as an assisted reproductive technology in animals and humans. This method needs high concentrations of cryoprotectants that can be toxic with high cooling degrees. Then, vitrification could be change histone modifications such as methylation and acetylation can performance as regulatory controls of gene transcription. So, the purpose of the presen...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Epigenetics & Chromatin
سال: 2013
ISSN: 1756-8935
DOI: 10.1186/1756-8935-6-s1-o11